please refer disclaimer overleaf. hifluoro pseudomonas hiveg agar base MV1469 hifluoro pseudomonas hiveg agar base is recommended for selective isolation of pseudomonas aeruginosa from clinical and non-clinical specimens by fluorogenic method. composition** ingredients gms / litre hiveg peptone no.2 18.000 magnesium chloride 1.400 potassium sulphate 10.000 cetrimide 0.300 fluorogenic mixture 2.050 agar 15.000 final ph ( at 25c) 7.20.2 **formula adjusted, standardized to suit performance parameters directions suspend 46.75 grams in 1000 ml distilled water containing 10ml glycerol. heat to boiling to dissolve the medium completely. sterilize by autoclaving at 15 lbs pressure (121c) for 15 minutes. mix well and pour into sterile petri plates. principle and interpretation pseudomonas aeruginosa (also known as pseudomonas pyocyanea ) is a gram-negative, aerobic, rod-shaped bacterium. like other pseudomonads, p. aeruginosa secretes a variety of pigments, including pyocyanin (blue-green), fluorescein (yellow-green and fluorescent), and pyorubin (red-brown). king et al developed pseudomonas agar p (i.e. king a media) for enhancing pyocyanin and pyorubin production and pseudomonas agar f (i.e. king b media) for enhancing fluorescein production (1). hifluoro pseudomonas hiveg agar base is prepared by completely replacing animal based peptones with veg peptones. this media is a modification of hifluoro pseudomonas agar base which is devised based on the formula described by king et al. (1) except fluorogenic mixture. it is used as the selective medium for the isolation of p. aeruginosa from pus, sputum and drains etc. cetrimide (cetyltrimethylammonium bromide) is incorporated in the medium to inhibit bacteria other than p. aeruginosa . it acts as a quaternary ammonium compound, cationic detergent that causes nitrogen and phosphorus to be released from bacterial cells other than p.aeruginosa . p.aeruginosa cleaves the fluorogenic compound to release the fluorogen which produces a visible fluorescence under long wave uv light. quality control appearance cream to yellow homogeneous f ree flowing powder gelling firm, comparable with 1.5% agar gel colour and clarity of prepared medium light amber colourec opalescent gel forms in petri plates reaction reaction of 4.675 % w/v aqueous solution at 25c. ph : 7.20.2 ph 7.00-7.40 cultural response cultural characteristics observed after an incubation at 35-37c for 18-24 hours. cultural response free datasheet http://
himedia laboratories technical data disclaimer : user must ensure suitability of the product(s) in their application prior to use. products conform solely to the information contained in this and other related himedia? publications. the information contained in this publication is based on our research and development work and is to the best of our knowledge true and accurate. himedia? laboratories pvt ltd reserves the right to make changes to specifications and information related to the products at any time. products are not intended for human or animal diagnostic or therapeutic use but for laboratory, research or further manufacturing use only, unless otherwise specified. statements contained herein should not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents. himedia laboratories pvt. ltd. a-516,swastik disha business park,via vadhani ind. est., lbs marg, mumbai-400086, india. customer care no.: 022-6147 1919 email: techhelp@himedialabs.com organism inoculum (cfu) growth recovery fluorescence (under uv) cultural response pseudomonas aeruginosa atcc 27853 50-100 luxuriant >=50% positive stenotrophomonas maltophila atcc 13637 >=103 inhibited 0% staphylococcus aureus atcc 25923 >=103 inhibited 0% escherichia coli atcc 25922 >=103 inhibited 0% storage and shelf life store dehydrated and prepared medium at 2-8c in tightly closed container. use before expiry period on the label. reference 1.king, ward and raney, 1954, j. lab. clin. med., 44:301. revision : 1 / 2011 free datasheet http://
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